The MagAttract Virus Mini M48 Kit can be used to purify nucleic acids from a broad range of DNA and RNA viruses. The yields of viral nucleic acids obtained in the purification procedure are normally below 1 ? and therefore quantitative amplification methods should be used to determine yields. The automated procedure gives more sensitive detection for the amount of starting material than other automated systems that use magnetic beads. Fully automated processing on the BioRobot M48 instrument provides highly reproducible purification, with minimal sample-to-sample or day-to-day variation. Parallel purification of 48 negative and positive (10? copies/ml armored RNA) samples with the MagAttract Virus Mini M48 Kit on the BioRobot M48 results in no detectable cross-contamination. Analysis was by real-time RT-PCR.
The MagAttract Virus Mini M48 Kit enables highly sensitive detection of a broad range of DNA and RNA viruses. Yields are linear, allowing accurate quantitative analysis for both low and high viral titers. Accurate detection requires cross-contamination-free processing to avoid false positives. The MagAttract Virus Mini M48 Kit on the BioRobot M48 processes samples with no detectable cross-contamination and is well-suited for purification of both high and low levels of viral nucleic acids from the same set of samples. Kit performance is not guaranteed for each virus species and has to be validated by the user.
The MagAttract Virus Mini M48 Kit provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum and plasma for highly sensitive detection in downstream assays. The kit can be used to purify nucleic acids that are free of proteins, nucleases, and other impurities from a broad range of DNA and RNA viruses for life science applications.
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For automated processing
BioRobot M48 Workstation
Sample tube (1.5 ml, 2 ml)
Main sample type
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein