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EuTc

Active Motif, Inc / Active Motif

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Overview

The EuTc-Hydrogen Peroxide Assay is based on a novel, Europium-based fluorescent reagent that is used to detect free or enzymatically produced H2O2. Europium tetracycline (EuTc) detects hydrogen peroxide in a fast and easy method, and without the need for additional enhancer solutions. This makes EuTc a sensitive reagent to monitor catalases, peroxidases or glucose-oxidase activities through in vitro enzymatic assays, or to study substances that inhibit or activate these enzymes.

Explanation

Hydrogen peroxide (H_O_) is a reactive metabolic by-product that is a key regulator in a number of oxidative stress-related states. Functioning through NFκB and other factors, hydroperoxide-mediated pathways have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down’s syndrome. Due to its implication in these many disease states, there is much interest in sensitive assays to monitor hydrogen peroxide in biochemical, clinical, as well as in environmental samples. In addition, there is much research on the enzymes that produce and eliminate hydrogen peroxide, and on their inhibitors.

Active Motif’s EuTc-Hydrogen Peroxide Assay is based on a novel fluorescent reagent that is used to detect free or enzymatically produced H_O_. Europium Tetracycline (EuTc) detects hydrogen peroxide in a fast and easy method, and without the need for additional enhancer solutions. This makes EuTc a sensitive reagent to monitor catalases, peroxidases or glucose-oxidase activities through in vitro enzymatic assays, or to study substances that inhibit or activate these enzymes.

The main advantages of using EuTc for luminescent peroxide detection are:
○Fast, 10-minute incubation time
○Applicable to turbid samples
○Functions at neutral pH
○No need for enzymatic amplification
○Large Stoke’s shift decreases interference of background fluorescence
○Long decay time enables time-resolved, or time-gated, measurement
Update:2015-08-20

Spec sheet

Cat. No. 15003
Unit Size 1000 rxns
Size
毒/劇
カルタヘナ
保管温度 室温
Price(JPY) 41,000

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